摘要: |
以IGFBP-3基因作为侯选基因,对秦川牛群体中IGFBP-3位点与秦川牛高档牛肉产量的关系进行了分析。结果发现,不同基因型对秦川牛高档牛肉产出率有一定影响,AA、AB及BB型个体的屠宰率、净肉率及西冷、牛柳、眼肉和嫩肩肉的产率逐渐降低,但差异不显著(P>0.05);AA型个体的眼肌面积大于BB型个体(P<0.05),AB型和BB型个体胴体脂肪含量高于AA型个体(P<0.01)。 |
关键词: 秦川牛 高档牛肉产量 胰岛素样生长因子结合蛋白-3(IGFBP-3)基因 PCR-RFLPs |
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基金项目:国家“863”计划项目(2001AA243052);国家自然科学基金项目(30070551);杨凌示范区专项基金(99KG8);农业部留学回国人员活动基金(1999-12D);西北农林科技大学青年基金资助项目(0808) |
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Polymorphisms of insulin-like growth factor binding protein-3 gene and its associations with several carcass traits in Qinchuan Cattle |
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Abstract: |
In this study,DNA samples from 13 Qinchuan cattle (Bos taurus) were analyzed with PCR-RFLPs This locus showed three genotypes,namely AA,AB and BB after electrophoresis.Frequencies of the genotype AA,AB,BB and allele A,B were 0.69,0.31,0 and 0.845,0.155,respectively.This polymorphic locus of IGFBP-3 gene was at Hardy-Weinberg equilibrium (P>0.05).The genotypes of AA,AB,BB slightly affected high quality beef cutting of Qinchuan cattle.Striplion percentage,Tenderloin percentage,Ribeye percentage and Tender shoulder percentage were decreased with the genotypes of AA,AB and BB in Qinchuan cattle,but it was not significant (P>0.05).Average ribeye area in individuals of AA genotype was significantly higher than that in individuals of BB genotype (P<0.05),and beef fat content in individuals of genotype AB and BB was significantly higher than that in individuals of AA genotype (P<0.01). |
Key words: Qinchuan cattle high quality beef cutting yield insulin-like growth factor binding protein-3 (IGFBP-3) gene DNA polymorphism |