| 摘要: |
| 为研究环腺苷酸(cAMP)对乳腺上皮细胞增殖的影响，建立了山羊乳腺上皮细胞培养体系。将细胞分为8个组，分别用1×10，1×102，1×103，1×104，1×105，1×106，1×107 pmol/mL cAMP刺激细胞，从中选出效果明显、组间差异显著的2组作为试验的高剂量组（1×107 pmol/mL）和低剂量组（1×106 pmol/mL）。从细胞群体倍增时间、细胞生长曲线和细胞分裂指数3个方面研究了cAMP对山羊乳腺上皮细胞增殖的影响。结果表明，低浓度的cAMP有促进细胞增殖的作用，高浓度cAMP有抑制细胞增殖的作用。 |
| 关键词: 山羊 乳腺上皮细胞 增殖 环腺苷酸 |
| DOI： |
| 分类号: |
| 基金项目:国家教委博士点基金资助项目（7914087） |
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| Effect of cAMP on proliferation in goat mammary epithelial cell |
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| Abstract: |
| Culture system in goat mammary epithelial cell for studing the effect of cAMP on proliferation in mammary epithelial cell was established.The cell was divided into 8 groups and stimulated by cAMP in 1×10，1×102,1×103,1×104,1×105,1×106 and 1×107 pmol/mL respectively.The two groups with tangible results and ramarkable difference among the groups were selected from the results.Then the two groups served as the higher dosage group (1×107 pmol/mL) and lower dosage group (1×106 pmol/mL) in this study.The effect of cAMP on proliferation in goat mammary epithelial cell was studed from the cell population doubling time,the cell growth curve and the cell division index.The results showed that the lower concentration of cAMP can promote the cell proliferation and the higher concentration of cAMP can inhibit the cell proliferation. |
| Key words: goat mammary epithelial cell culture system cAMP |
