| 摘要: |
| 应用PCR-Amp-FLP技术对75例陕西正常老年人群、94例老年动脉粥样硬化(AS)患者与71例陕西正常青年人群的YNZ22-VNTR(可变数目串联重复序列variable number tandem repeats)位点进行多态性分析。结果显示，在正常青年人群中YNZ22位点共检测出29种基因型，10个等位基因，其片段大小在168～938 bp，杂合度为70.4％，多态信息量(po1ymorphism information content，PIC)为0.84；老年人群中YNZ22基因点共检测出20种基因型，9个等位基因，其片段大小在168～798 bp，杂合度为24％，多态信息量(PIC)为0.84，与青年群体的基因频率分布有显著差异；动脉粥样硬化(AS)人群中YNZ22基因点共检测出23种基因型，9个等位基因，其片段大小在168～728 bP，杂合度为24.47％，多态信息量为0.81。表明老年群体杂合度显著降低，员主要的是在基因型数目与大基因片段上老年群体与育年群体差异显著。YNZ22位点多态性改变或不稳定可能是引起衰老的原因之一。 |
| 关键词: YNZ22基因 老年人群 衰老 数目可变串联重复序列 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39700165) |
|
| Analysis on genetic polymorphism of YNZ22 VNTR in old and youth population |
|
|
| Abstract: |
| The polymorphism at YNZ22-VNTR locus was studied by PCR-Amp-FLP in 71 cases of Youth,94 cases of atherosclerosis and 75 cases of normal old people of Shaanxi.Results show that:① 29 genotypes and 10 alleles ranging from 168 bp to 938 bp were detected in the 71 normal youth subjects.② 20 genotypes,9 alleles ranging from 168bp to 798bp were revealed in normal old people of Shaanxi.The heterozygosity of YNZ22 was 24%,PIC 0.84;③ 23 genotypes,9 alleles ranging from 168 bp to 728 bp were revealed in atherosclerosis cases.The heterozygosity of YNZ22 was 0.24,PIC 0.81.④ There is obvious difference in distribution of allele frequency in two groups (Old and Youth);and the heterozygosity of old people decreases obviously.There is obvious difference in the number of genotypes and large alleles in two groups.⑤ Our results suggest that unstability of YNZ22 locus can be related to senility. |
| Key words: YNZ22 gene old population senility variable number tandem repetition |
