| 摘要: |
| 利用花粉管通道法，再次将大豆DNA导入在生产上具有应用前景的粳稻97冬繁10；测定了在常规施肥基础上，后期追施尿素氮肥、整个生育时期只施有机肥和常规施肥3种施肥方式下种植的97冬繁10、导入大豆DNA后的97冬繁10、97冬繁13和优丰4种水稻的糙米蛋白质含量。结果显示：①在高氮、有机和常规3种施肥方式下，已导入大豆DNA的97冬繁10水稻糙米蛋白质平均含量依次为128.0,123.7和102.1 mg/g，分别比相同上对照提高47.3,48.0和33.3 mg/g；②虽然高氮和有机栽培的稻米蛋白质平均含量都比常规栽培稻米高，但4种水稻糙米蛋白质平均含量增加程度及变异范围不同。经大豆DNA处理的水稻在高氮和有机栽培条件下，糙米蛋白质平均含量增加最明显，而且变异范围明显加宽。本研究结果进一步说明，利用花粉管通道法导入大豆DNA能够提高水稻糙米蛋白质含量，并且后期追施氮肥和早期施用有机肥栽培，更有利于其糙米蛋白质含量的表达。 |
| 关键词: 水稻 外源DNA导入 糙米蛋白质含量 施肥方式 |
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| 基金项目:上海市科学技术发展基金项目(983113008) |
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| Effect of different fertilizer application on crude protein contents in variation rice seeds induced by exogenous DNA from soybean |
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| Abstract: |
| The exogenous DNA from soybean was introduced into rice seeds through the pollen-tubepathway again.Three kinds of culture was were designed:the culture in high nitrogenous fertilizer,the culture in organic fertilizer,the culture in conventional fertilizer.Four kinds of rice-two ordinary varietes,the variety induced by exogenous DNA from soybean and the contrast were respecitively panted with the three kinds of culture ways.Crude protein contents in the four kinds of rice offsping’s seeds were analyzed.The results indicated:(1)The average contents of crude protein in the seeds induced by exogenous DNA from soybean and planted in the high nitrogenous fertilizer,i the organic fertilizer and conventional culture were 128.0 mg/g,123.7 mg/g and 102.1 mg/g,increasing separately by 473.0 mg/g,479.7 mg/g and 332.9 mg/g over their contrasts planted in relevant soils;(2)Variable range of crude protein contents in the variation rice seeds was wider in high nitrogenous fertilizer culture and in organic fertilizer culture than in the convertional fertilizer.This research result confirms that introducing exogenous DNA through the pollen-tube pathway could increase crude protein content in rice seeds.The culture way in high nitrogenous fertilizer and in organic fertilizer are advantageous to expression of crud protein content in the variation rice seeds. |
| Key words: rice soybean exogenous DNA introduction crude protein content fertilizer application pattern |
