| 摘要: |
| 研究了潮霉素不同质量浓度对西农1376和京花1号小麦愈伤组织及种子的筛选效果。结果表明，潮霉素对小麦愈伤组织的适宜筛选质量浓度为110 mg/L，对转基因小麦后代种子筛选以140 mg/L较为适宜；不同基因型材料对潮霉素的敏感性存在一定差异。 |
| 关键词: 小麦 遗传转化 潮霉素 |
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| 基金项目:国家重点科技(攻关)项目 (96-009-01-14)，华中农业大学作物遗传改良国家重点实验室开放课题 (2000-36) |
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| Study on the optimal mass concentration of hygromycin in wheat transformation |
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| Abstract: |
| The selection effects on the wheat immature embryo calli and the seed of Xinong 1376 and Jinghua 1 with different mass concentration of hygromycin were studied.The results showed that the optimal mass concentration of hygromycin was 110 mg/L to wheat immature embryo calli selection and 140 mg/L to wheat seed.There was a little difference of different wheat genotype to hygromycin. |
| Key words: wheat genetic transformation hygromycin |
