| 摘要: |
| 为Dx5基因设计了1对特异引物，选用HMW-GS在GluDxl位点已知的22个材料对其进行了验证。结果表明，该引物的准确率达到100％，完全可以作为Dx5基因的检测引物。利用这1对引物，通过PCR技术对40种材料的Dx5基因进行了检测，发现仅有98605，陕253，郑州891,97-2143，绵阳96171-10，绵阳19，中1813－1和绵阳11等8个品种（系）携带Dx5基因，占待测材料总数的20.0%，远远低于北美小麦品种中Dx5基因的携带率。研究中还发现，对检测优质面包烘烤品质基因而言，PCR方法是最简便、快速、准确的方法之一。 |
| 关键词: 小麦品质 PCR技术 Dx5基因 |
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| 基金项目:杨凌农业生物技术育种中心项目(2001B-5)，北京大学蛋白质工程及植物基因工程实验室开放项目 (1999-7)，法国利马格兰小麦合作项目(1999CW-2 ) |
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| PCR-detection of high molecular weight glutenin subunit Dx5 gene in wheat |
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| Abstract: |
| A pair of Dx5 gene primers were designed on the basis of difference between the Dx5 gene and the Dx2 gene.The 22 materials whose HMW-GS Glu-Dx1 locus was known were detected with PCR-based approach in this paper.The results showed that the degree of accuracy was 100% using the primers designed for Dx5 gene.It is useful for the primers designed for the primers of Dx5 gene to detect Dx5 gene in wheat lines.The Dx5 genes of 40 Chinese strains were detected with PCR based on the primers above.The results showed the Dx5 gene was found in 8 strains included 9860-5,Shaan 253,Zhengzhou 891,97-2143,Mianyang 96171-10,Mianyang 19,Zheng 1813-1,Mianyang 11.The probability of the Dx5 gene that is 20.0% in Chinese wheat lines is far lower than in North America wheat lines.This easy,quick PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting bread wheat genotypes with good bread-making properties. |
| Key words: wheat polymerase chain reaction(PCR) Dx5 gene |
