| 摘要: |
| 对采自云南各大烟区的烟草野火病菌株进行了分离、鉴定，用通过筛选致病力强的烟草野火病菌株为抗原，制备了烟草野火病菌特异性的抗血清，建立了一套快速检测烟草野火病菌的方法，制备了SPA－ELISA反庆试剂盒，应用玻片凝集或协同凝集反应与SPA－ELISA反应试剂盒相结合的方法，使检测真正做到了简便、快速、灵敏、准确。 |
| 关键词: 烟草野火病 烟草野火病菌 快速检测 间接-ELISA SPA-ELISA 凝集反应 |
| DOI： |
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| 基金项目:云南省烟草公司基金项目(98A55) |
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| Studies on rapid detection of tobacco wildfire (Pseudomonas syringae pv.tabaci) in Yunnan |
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| Abstract: |
| Strains of tobacco wildfire from Yunnan were isolated and identified.Pseudomonas sytingae pv. tabaci specific antiserum was obtained through screening the strong-virulent strains as antigen. Straphyloclcal protein A ELISE(SPA-ELISA) kits were developed successfully and this was the first time to establish the rapod detecting method of the pathogen.Detection techniques become easieer,rapoder,more sensitive and accurate,by combining SPA-ELISA with agglutination test(slide agglutination and coagglutination). |
| Key words: tobacco wildfire Pseudomonas syringae pv.tabaci rapid detection indirect-ELISA,SPA-ELISA agglutination |
