| 摘要: |
| 从260个随机引物中筛选出多态性丰富的25个引物进行中国野生葡萄商－24与欧洲葡萄龙眼种间杂交F1代的RAPD分析。据对219个RAPD标记的统计分析结果表明，不分离标记、按1：1或3：1比例正常分离的标记、偏离孟德尔分离比例的标记和异常分离的标记分别占49.8％，40.6％，7.3％和2.3％。双亲共有标记中75.0％不发生分离，是不分离标记的主体。单亲特有标记中55.4％～65.3％发生正常分离，是1：1分离标记的主体。商－24特有标记中不分离标记占20.4％，而龙眼特有标记中不分离标记占36.9％，商－24基因组中纯合基因位点比例要低于龙眼基因组中纯合基因位点的比较。异常分离的标记是指非亲本RAPD标记，本研究出现了5个异常分离的标记。符合1：1或者3：1分离比例的特有RAPD标记，可分别用于构建商－24和龙眼的遗传连锁图谱，双亲共有标记可用于构建双亲的遗传连锁图谱。 |
| 关键词: 葡萄 种间杂交 RAPD分析 |
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| 基金项目:国家自然科学基金资助项目(39770525,39970524)；高等学校博士点基金资助项目(980701)；教育部资助年轻优秀教师基金项目 |
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| RAPD analysis in an interspecific F1 hybrid of grapes |
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| Abstract: |
| RAPD analysis in an interspecific F1 hybrids of grapes was studied using 25 primers screening from 260 oligonucleotide random primers.According to the segregation patterns,RAPD markers were grouped into three types:Normal Mendelian inheritance with Segregation ratio of nearly 1∶1 (as a result of Aa×aa or aa×Aa),3∶1(as a result of Aa×Aa) or 1∶0 (non-segregation,as result of AA×AA,AA×aa,AA×Aa,Aa×AA and aa×AA),deviation from Mendelian segregation ratios,non-parental markers,absent in both parents but present in progenies.Basing on statistics of 219 RAPD markers,there exist 49.8% non-segregation markers,40.6% 1∶1 and 3∶1 Mendelian segregation markers,7.3% markers deviating from Mendelian segregation ratios and 2.3% abnormal segregation markers.75.0% markers presenting in both parents did not segregate and 55.4%-65.3% markers presenting in only one parent show Mendelian segregation.Non-segregation markers accounted for 20.4% of the markers presented only in Shang-24 and 36.9% of those presented only in Longyan,the results suggested that Longyan genome contains more pure loci than Shang-24.These provide the foundation for construction of grape genetic linkage map. |
| Key words: grape interspecific hybridization RAPD analysis |
