| 摘要: |
| 根据无核基因特异APD标记的序列进行分析，合成1对特异引物P1＋P3（序列分别是P1：5＇CTC ATC TTC TTG ATG GTG AT 3＇；P3：5＇AAG TGA AGA ACA TCA TTA AGA AC 3＇），对30个葡萄材料进行PCR扩增，成功地将RAPD标记转换成SCAR标记。并对含有该标记序列的重组质粒进行Hind Ⅲ和Spe I双酶切，获得的310 bp片段回收后制成探测葡萄无核基因存在与否的杂交探针，成功地对7个葡萄品种进行Southern blot分析，实现了分子标记检测葡萄无核基因的目的。 |
| 关键词: 葡萄无核基因 SCAR标记 Southern blot分析 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39970524)；农业部948项目(981043) |
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| SCAR marker linked to seedless genes in grapes and southern blot analysis |
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| Abstract: |
| This paper reported a rapid detection of genetic marker linked to seedless genes in 30 grape varities using SCAR marker and Southern blot analysis.One pair of specific primers were designed based on the sequence analysis of the RAPD marker linked to seedless genes in grapes,and synthesized to perform PCR.The RAPD marker was transformed into SCAR marker successfully.Two restriction enzymes were selected to digest recombinant plasmids and the genome DNA of seven seeded and seedless grape cultivars.The 310 bp specific fragment obtained from recombinant plasmids was made into a probe after DIG labling to detect the seedless genes in grapes.The digested genome DNA showed the existence of the seedless genes in seedless cultivars using southern blot,whereas the seeded ones were not. |
| Key words: seedless gene in grape SCAR marker southern blot |
