| 摘要: |
| 用MTT法测定了CD58对山羊妊娠不同时期子宫内膜γδT细胞的活化作用,同时应用ELISA法,分别测定了山羊妊娠第30天的子宫局部γδT细胞在体外活化后分泌TGF-βs的特性.结果表明,CD58可以剂量依赖的方式刺激γδT细胞活化,在试验剂量范围内,1.25 mg/L CD58刺激作用最强.同时,各刺激因素的作用在妊娠的不同时期也存在差异,在相同剂量CD58的刺激下,γδT细胞的活化程度自妊娠第20天起至妊娠3个月,依次升高,4个月又呈下降趋势.PHA-P和CD58均可刺激子宫内膜γδT细胞分泌TGF-β1和TGF-β2,其分泌量与细胞的活化程度一致.与目前已研究的小鼠等动物不同,TGF-β1的分泌量显著高于TGF-β2. |
| 关键词: 山羊 γδT TGF-βs 妊娠 |
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| 基金项目:国家自然科学基金资助项目(39970544) |
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| Abstract: |
| The research of the effects of CD58 on the activation and its secretion of γδT in endometrial lymphocytes,which play a major role in immunological enduration during gestation,has significance on revealing the effects of CD58 on the local immunological regulation during gestation.In the present study,the effects of CD58 on activation of the γδT cells prepared by magnetic cell sorting (MACs) from goat endometrial lymphocytes during the certain time of gestation were determined with the method of MTT.Further,the secreting activities of TGF-βs of the proliferated γδT cells obtained from the goat 30 d after gestation were investigated.The results indicate that CD58 was able to activate the γδT cells in a dose-dependent manner (P＜0.05)，in which the dose of 1.25 mg/L of CD58 had the most significant effect within the doses used in the tests（P＜0.01).The effect enhanced with the development of gestation from the 20th day to 3 months during pregnancy,and downtrend appeared after 4 months.Both PHA-P and CD58 are able to stimulate the γδT cells to secrete TGF-β1and TGF-β2,the amount of which is consistent with the activity of the γδT cells.While inconsistent with the result on mouse,the amount of TGF-β1 is higher than TGF-β2. |
| Key words: goat γδT TGF-βs pregnancy |
