摘要: |
为了探索成都麻羊的品种特点和群体遗传结构,应用随机抽样方法,调查了四川省大邑县的42只成都麻羊,用淀粉凝胶电泳法测定了33个血液蛋白座位,并对其外形特征进行了检测。 结果表明,运铁蛋白(Tf)、亮氨酸胺肽酶(LAP)、碱性磷酸酶(Alp)、前白蛋白-3(Pa-3)、淀粉酶(Amy)和酯酶-D(Es-D)6个血液蛋白座位表现多态性,多态位点比例为0.181 8;11个类别25种外型特征中8个类别有变异体。平均杂合度分析表明,成都麻羊变异程度较小(H=0.054 5),结合现实实际调查情况,可以认为成都麻羊群体有效规模较小,分布地域狭小,急需保种。 |
关键词: 成都麻羊 血液蛋白多态性 外型特征 遗传检测 |
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基金项目:国家自然科学基金资助项目(38370507) |
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Studies on genetic polymorphism of Chengdu Grey Goat |
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Abstract: |
In order to clarify the variety characteristics and genetic structure of Chengdu Grey Goat,the genetic examination was carried out with 33 blood protein loci examined by starch gel electrophoresis and 11 appearance features by simple random sampling in Sichuan Province Dayi County.The gene frequencies of blood protein loci and the phenotypic frequencies of appearance feature were estimated.The results showed that polymorphism was found at six blood loci of Tf,Lap,Alp,PA-3,Amy and Es-D,the proportion of polymorphous loci (Ppoly) is 0.181 8,and that variant was found at 7 appearance features.And the result of average heterozygosity(H=0.054 5)analysis indicated that Chengdu Grey Goat had little variability and small effective size of population,and had faced the sense of urgency of breed preservation. |
Key words: Chengdu Grey Goat blood protein polymorphism appearance feature genetic examination |