| 摘要: |
| 采用反转录PCR（RT-PCR）和套式PCR（nest Polymerase Chain Reaction,nPCR）扩增出3株广西省近期（1999～2000年）猪瘟流行野毒的E2基因，将其分别克隆于PMD-18T载体上并进行了核苷酸序列测定，根据C株及Brescia和Alfort株确定起始氨基酸三联体的正确位置后进行氨基酸序列推导，同时进行了同源性比较及E2糖蛋白结构的分析。结果表明，所测3株HCVE2基因的长度均为1 170bp，编码的氨基酸序列均包括完整信号肽序列和部分跨膜序列，共由384个氨基酸组成。3株流行毒的E2基因核苷酸序列同源性为90.10%～98.54%,相应的氨基酸序列同源性为89.59%～97.92%。这3株流行毒与C-株兔脾组织毒（疫苗种毒）E2基因的核苷酸序列同源性为82.87%～83.99%，相应的氨基酸序列同源性为86.98%～90.10%,表明近期猪瘟流行毒与C-株疫苗毒的gp55蛋白之间存在一定的差异。 |
| 关键词: 猪瘟病毒 E2基因 糖蛋白 序列分析 |
| DOI： |
| 分类号:TQ464.7 |
| 基金项目:国家B类攀登计划项目(85-44) |
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| Amino-acid difference in the main antigen domain GP55(E2) of the C-strain and the Guangxi prevalent strains hog cholera virus(HCV) |
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ZHAO Ji-qiang 1 LI Xia 2 CHENG Zhi-hui 1 CHEN Hang 2 LI Yuan 3 CHEN Song-sen 3
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| Abstract: |
| The E2 gene of three prevalent virulent strains of Hog Cholera Virus (HCV) from Guangxi China were amplified by reverse transcription (RT) and the nested Polymerase Chain Reaction (nPCR).The amplifed E2 fragments of three HCV strains were all 1 170 bp in length by agarose gel electrophoresis.Three E2 fragments were cloned respectively into PMD-18T vector.1 170 bp cDNA fragments of three prevalent virulent E2 gene were sequenced,and based on the amino acid sequences of C-strain,Brescia and Alfort strain 384 residues,amino acid sequences of E2 were deduced.The nucleotide homology among three prevalents virulents were 90.10% to 98.54％;and that of amino acid were 89.59％ to 97.92％.The nucleotide identity of C-strain from spleen tissue of rabbit with that of three Chinese prevalent virulent strains were 82.87％ to 83.99％,and that of amino acid were 86.98％ to 90.10％.This showed that there was some differences between gp55 of C-strain and the 3 prevalent virulent strains. |
| Key words: hog cholera virus E2 gene glycoprotein sequence analysis |
