| 摘要: |
| 用完全福氏佐剂对14只家兔进行基础免疫10 d后，将家兔随机分成2组，每组7只，1组为苦马豆素-BSA免疫组，另1组为对照。首免时免疫组每只兔四足掌共注射0.8 mL，两侧大腿内侧肌肉各注射0.3 mL，两侧颈部皮下各注射0.3 mL，免疫总剂量为2 mL/只，抗原含量2 mg/mL。5周后第1次加强免疫，免疫组每只兔两侧大腿内侧肌肉各注射0.5 mL，背部皮下4点注射，每点0.25 mL，免疫总剂量为2 mL/只，抗原含量3 mg/mL。1周后试血。10 d后第2次加强免疫，免疫途径与第1次加强免疫相同，免疫总剂量为2 mL/只，抗原含量6 mg/mL。1周后试血。结果表明，苦马豆素-BSA第1次加强免疫1周后，1-7号兔都产生了抗体，间接血凝试验测定的抗体滴度分别为21,21,22,21,21,22和22，间接ELISA试验测定的抗体滴度分别为22,22,23,22,22,23和23。第2次加强免疫1周后试血，1-7号兔抗体滴度都明显升高，间接血凝试验测定的抗体滴度分别为26,26,28,27,27,28和28，间接ELISA试验测定的抗体滴度分别为27,27,29,28,28,29和29。 |
| 关键词: 苦马豆素 免疫原 家兔 |
| DOI： |
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| 基金项目:国际科学基金资助项目(IFS,No2906-1);国家自然科学基金资助项目(39770571) |
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| Study on immunogenicity of swainsonine-BSA |
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| Abstract: |
| After basically immunized with complete Freund’s adjuvant,14 rabbits were divided into 2 groups equally.Group one was immunized with swainsonine-BSA,which was used as the vaccine after emulsifying it with complete Freund’s adjuvant.The other was the control group.Immune procedure was as followings.The rabbit was injected 2 mL swainsonine-BSA vaccine,containing 4 mg swainsonine-BSA.0.8 mL was injected into 4 plantae,each fore-plantae was 0.15 mL and hind-plantae was 0.25 mL.0.6 mL was injected inside hind-leg,with each leg 0.3 mL,i.m. 0.6 mL was injected into neck,i.p. After 5 weeks the first intensive immunization was conducted,swainsonine-BSA was dissolved in water for injection.2 mL of the solution,containing 6 mg swainsonine-BSA,was injected to the rabbit.1 mL was injected inside hindleg,each leg 0.3 mL,i.m. 1 mL was injected 4 points of back,0.25 mL in each point.Antibody was monitored after a week.The second intensive immunization was conducted after 10 days.Immune dose was 2 mL,containing 12 mg swainsonine-BSA.Immune way was the same as the first intensive immunization.Antibody was monitored after a week.Immunoassay was conducted with indirect hemagglutination test and indirect ELISA test.After a week of the intensive immunization,all rabbits produced antibody.The titres,monitored by indirect hemagglutination test,were 21,21,22,21,21,22,,and indirect ELISA test,were 22,22,23,22,22,23 and 23.After a week of the second intensive immunization,the titres,monitored by indirect hemagglutination test,were 26,26,28,27,27,28 and 28,and indirect ELISA test,were 27,27,29,28,28,29 and 29. |
| Key words: swainsonine immunogen rabbit |
