摘要: |
采用RT-PCR技术对关中奶山羊不同大小的有腔卵泡的卵泡膜细胞、蜞粒细胞和卵丘卵母细胞复合体中转化生长因子β2(TGFβ2)的基因表达进行了测定。结果表明,在有腔卵泡的各个时期都有TGFβ2基因表示;在卵泡膜、颗粒细胞和卵丘卵母细胞复合体中也检测到了该生长因子的mRNA。推测TGFβ2对关中奶山羊卵泡发育有一定的调节作用。 |
关键词: 山羊 有腔卵泡 TGFβ2 基因表达 |
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基金项目:国家自然科学基金资助项目(39830280) |
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The gene expressin of TGFβ2 in goat antral follicles |
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Abstract: |
The gene expression of transforming growth factorβ2(TGFβ2) in goat antral follicales was dtermined by utilizing reverse transcriptase-polymerase chain reaction (RT-PCR).In different size of goat ovarizn follicles and in theca cells,granular cells (GCs) and cumulus-oocyte complexes (COCs),the expression of mRNA of TGFβ2 can all be measured.These results indicate that TGFβ2 may play an important role in goat ovarian follicle development and oocyte maturation. |
Key words: goat antral follicles TGFβ2 gene expression |