| 摘要: |
| 从全能性或多能性细胞获取，分化抑制物选择，培养基，分离方法，鉴定，保存等6个方面，对胚胎干细胞的分离培养体系的建立进行了系统综述。 |
| 关键词: 胚胎干细胞 分离 克隆 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39670359);国家攻关资助项目(96-C01-03) |
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| Isolation and cloning of embryonic stem cells (ES cells) |
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| Abstract: |
| In this paper,we reviewed the establishment of ES cells culture system in following aspects:obtaining of totipotent (or pluripotent) cells,selection of inhibitory substances,media,isolation methods,examination and freeze preservation. |
| Key words: ES cells isolation cloning |
