| 摘要: |
| 用25种随机引物对苹果，梨，杏，酸枣，枣，山楂6种寄主桃蛀果蛾基因组DNA进行了随机扩增多态DNA（RAPD）分析，结果表明，25种随机引物中有22种出现不同程序的DNA多态性标记，多态多百比为71.9％，根据Nei氏片段共享度公式计算了6种寄主桃蛀果蛾两两配对间的遗传距离，6种寄主桃蛀果蛾的平均遗传距离为0.542 6，其中梨桃蛀果蛾与酸枣桃蛀果蛾传距离最远为0.704，枣与酸枣传距离最近为0.4627，用UPGMA法构建了6种寄主桃蛀果蛾的分子聚类图。 |
| 关键词: 桃蛀果蛾 RAPD-PCR 遗传变异 寄主 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目 |
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| RAPD-PCR analysis of genetic variation of Carposina sasakii Matsumura(Lepidoptera Carposinidae) in different host plants |
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| Abstract: |
| In this research,RAPD-PCR was adopted to analyse the genomic DNA of Carposina sasakii Matsumura of 6 host plants (apple,pear,apricot,jujube,wild-jujube,hawthorn) with 25 random primers.As a result,22 of the 25 random primers showed variable polymorphic marks with the polymorphic frequency of 71.9%. Based on these results,we calculated the genetic distance of 6 populations.The average genetic distance of 6 populations was 0.542 6,of which the most distant is between wild-jujube and pear,and the nearest is between jujube and wild-jujube.At the same time,the molecular dendrogram of the 6 populations was constructed with UPGMA method. |
| Key words: Carposina sasakii Matsumura RAPD-PCR genetic variation host |
