| 摘要: |
| 鸭源NDV-D10毒株与EDS-76GC2毒株混合接种同一鸭胚，培养96 h收集尿囊液，制成ND-EDS-76异源二联油乳剂灭活疫苗，然后与普通二联油乳剂灭活疫苗接种同一鸡群，在10，20，30，180，360 d分别检测抗体滴并进行比较，结果差异不显著，在免疫后30，180，360 d随机取鸡各60只为1组，用NDV-F48E9和EDS-76GC2强毒株进行攻毒保护试验并进行比较，结果表明两种疫苗在30，180 d免疫保护率均为100％，360 d对ND76异源二联油乳剂灭活疫苗，可有效地保护整个产蛋期对ND和EDS-76强毒的攻击。 |
| 关键词: ND-EDS-76异源二联油苗 田间对比试验 抗体滴度 免疫保护率 |
| DOI： |
| 分类号: |
| 基金项目:天津市自然科学基金资助项目(973611911) |
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| Field test on oil emulsion inactive duck origin ND-ESD-76 bivalent combinet vaccine |
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| Abstract: |
| Duck origin NDV-D10 and EDS-76GC2 virus strains were propagated by the same embryos and harvested from embryo allantoic fluid after 96 h culture,and used to prepare oil emulsion inactivated ND-EDS-76 bivalent combined vaccine.Two groups of chicken were vaccinated with the newly developed vaccine and ordinary oil emulsion inactivated bivalend combined vaccine respectively,and the difference of antibody titres between the two groups were not significant.The challenge NDV-F48E9 and EDS-76GC2 virulent strains both acted at 30,180 and 360 d post-immunization.The two vaccinated groups with 60 birds each,selected randomly,showed 100% protectivity at the first two challenge and over 97% and 87% protectivity at last challenge,but not significant difference.The clinical inoculation of layers aged about 120 d with oil emulsion inactivated duck origin ND-EDS-76 bivalent combined vaccine to 2 million single chicken could obtain excellent immune effects of prevent two corresponding field virulent virus infection throughout the laying period. |
| Key words: oil emulsion inactivated duck origin ND-EDS-76 bivalent combined vaccine field-comparison test antibody titre immune protective rate |
