| 摘要: |
| 对山羊不同发育阶段卵母细胞OPS玻璃化冷冻的超微结构损伤进行了电镜观察，结果表明，卵丘细胞除内质网扩张外，未见其他明显的结构损伤，卵母细胞的质膜损伤以GV期最为严重，其次为培养9 h的，IVM的最轻，GV期卵几乎看不到微绒毛，而培养9h和IVM卵微绒毛保存较好，GV期卵母细胞线粒体有的发生了损伤，主要表现在嵴不清或扩张，培养9 hn和IVM卵母细胞的线粒体基本上保持了正常的结构，培养9h卵母细胞的内质网发生了扩张，IVM卵母细胞内质网结构正常。 |
| 关键词: 卵母细胞 OPS玻璃化 超微结构 山羊 |
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| 基金项目:成都大熊猫繁育研究基金(9820);国家计划与发展委员会资助项目(1996-2277) |
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| Uitrastructural changes in goat oocytes cryopreserved by OPS vitrification |
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| Abstract: |
| Ultrastructural damage of goat oocytes at different maturation stage was investigated after OPS vitrification procedure using transmisson electron microscopy (TEM). Cumulus cells keep normal structure except the swelling of endoplasmic reticulum (ER).The oolemma was most seriously damaged in GV oocytes,the second in 9 h,the third in IVM.Microvilli in GV oocytes was often absent,but in 9 h and IVM oocytes,microvilli was well-preserved.Swelling of mitochondrial cristae was seen in GV oocytes.Mitochondriar in 9 h and IVM oocytes was basically well-preserved.Swelling of ER was seen in 9 h oocytes,ER was well-preserved in IVM oocytes. |
| Key words: oocytes OPS vitrification ultrastructure goat |
