| 摘要: |
| 将鸭源鸡新城疫（ND）病毒D10株和鸡减蛋综合症（EDS-79）病毒GC2株同时接种于同一鸭胚内延长制，增殖96 h收取尿囊液，用甲醛灭活后加白油佐剂制成ND-EDS-76异源二联油乳剂灭活疫苗。该疫苗生产工艺简单，成本低，经试用表明可有效地预防ND和EDS-76的发生。 |
| 关键词: 鸡新城疫 鸡减蛋综合症 异源二联油乳剂灭活疫苗 油苗研制 |
| DOI： |
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| 基金项目:天津市自然科学基金资助项目（933611911） |
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| Study on oil emulison inactivated duck origin ND-EDS-76 bivalent combined vaccine |
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| Abstract: |
| Duck origin ND.D10 strain and EDS-76GC2 strain were inoculated into the same duck embryos and harvested after96hours of culture.The propagated virus were inactivated by formalin and mingled with oil emulsion adjuvant according to rational rate,and a combined inactivated oil-adjuvant duck origin NDV and EDS-76 vaccine was developed,which simplified the production process and reduced production cost.The tests of vaccine safety,antibody response,protective rate and duration of immunity were also preformed.The results show that this kind of vaccine could prevent ND virus and EDS-76 virus infection successfully. |
| Key words: newcastle disease,egg drop syndrome(EDS-76) oil emulsion inactivated duck origin ND-EDS-76 bivalend vaccin oil emulision production, |
