摘要: |
对平邑甜茶叶盘离体再生条件进行了研究,结果表明,平邑甜茶叶盘愈伤和分化适宜的BA和IBA质量浓度分别为0.5~1.0和0.2mg/L,愈伤至少需黑暗培养35d;茎尖分化的适宜培养基为MS附加BA0.5mg/L、IBA0.1~0.2 mg/L;适宜的生根培养基为1/2MS附加IBA0.1mg/L。 |
关键词: 平邑甜茶,组织培养,植株再生,叶盘法 |
DOI: |
分类号:S571.103.53 |
基金项目:西北农业大学青年教师科研基金资助项目 |
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Study on the regeneration of Malus hupehensis Rehd. |
ZHANGJun-ke
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Abstract: |
Conditions influencing regeneration by leaf disk of Pingyitiancha (Malus hupehensis hd.) were studied.The result showed that the optimum concentrations of benzyladenine (BA) and Indole-3 butyric (IBA) for Pingyitiancha leaf disk callus inducing and differentiation were 0.5-1.0 mg/L and 0.2 mg/L respectively.35-days dark culture was needed during callus inducing.The optimum medium for shoot tip differentiation was MS supplemented with BA 0.5 mg/L and IBA 0.1-0.2 mg/L.The optimum medium for rooting was 1/2MS supplemented with IBA 0.1 mg/L. |
Key words: Malus hupehensis Rehd.,tissue culture,plant regeneration,leaf disk method |