| 摘要: |
| 对用SDS法、尿素法、改良SDS法、氯化苄和CTAB法提取的大白菜基因组DNA进行了比较。结果表明,改良SDS法提取的DNA具有典型的天然DNA分子的标准紫外吸收光谱特点,其A260/A280为1.7～1.9,A260/A230为1.8～2.0,叶片的DNA产量为400μg/g。用该法提取的大白菜DNA适于进行RAPD分析。 |
| 关键词: 大白菜,DNA提取方法,改良SDS法 |
| DOI： |
| 分类号:S634.1;;Q781 |
| 基金项目:高等学校博士学科点专项科研基金项目(960701) |
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| Comparison of genomic DNA extraction methods of Chinese cabbage (Brassica campestris ssp.pekinensis) |
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WANGYong-fei
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| Abstract: |
| The DNA samples of Chinese cabbage male sterile line“CMS3411-7”and maintainer line“3411-7”were prepared by SDS,Urea, improved SDS, Benzyl chloride and CTAB methods, respectively.The results showed that DNA samples obtained by the improved SDS method possessed the standard ultraviolet absorbance spectrum of pure natural DNA,whose A260/A280 was 1.70-1.90 and A260/A230 was 1.8-2.0.The yield of DNA obtained by the improved SDS method was 400μg/g young leaves, and the DNA samples prepared by this procedure were suitable for RAPD analysis. |
| Key words: Chinese cabbage ( Brassica campestris ssp.pekinensis ),DNA extraction method,improved SDS |
