| 摘要: |
| 选用经高温灭活的香菇原养型双核原生质体作供体亲本 ,以带有脯氨酸( Prol)营养缺陷型标记的凤尾菇单核原生质体为受体亲本 ,对其原生质体融合情况进行研究。结果发现 ,两亲本原生质体的融合再生率为 0.106%,部分融合子菌丝细胞多核 ,无锁状联合。对该融合子再进行原生质体分离、再生 ,得到了具有新的生理特性的菌株材料。表明这种单双核原生质体融合是实现食用菌属间远缘杂交育种的有益探索 |
| 关键词: 香菇,凤尾菇,原生质体,远缘杂交育种 |
| DOI： |
| 分类号:S646.12 |
| 基金项目:河南省科技攻关项目(981050019) |
|
| A preliminary study on intergeneric protoplast fusion between Lentinus edodes and Pleurotus sajor-caju |
|
MIAO Yan-fang
|
| Abstract: |
| The protoplasts of wild dikaryon of L.edoes were heat-inactivated as dead donor and the protoplasts of auxotrophic mutan Monokaryon of P.sajor-caju were used as recipient.A study of two parents fusion showed that the regeneration ratio of fusion was 0.106%.Some of the fusants were polyenergid,but no clamp connection.Through protoplast isolation and reversion of one polyenergid fusant,new strains with new physiological characteristics were obtained,such as fast mycelial growth and earlier fruiting.The experiment result showed that the technique and method used in this study might be used as profitable exploration for distant hybridization. |
| Key words: Lentinus edodes,Pleurotus sajor-caju,protoplast,distant hybridization |
