| 摘要: |
| 用含体积分数0. 5%血清的DMEM直接饥饿胎儿及皮肤成纤维细胞,细胞数先是略有增长,然后逐渐下降。胎儿和皮肤成纤维细胞在血清梯度饥饿期间,更换含体积分数为4%, 2%和1%血清的DMEM,细胞仍缓慢增长;更换含体积分数为0. 5%血清的培养液后,细胞数逐渐降低。血清饥饿成纤维细胞后,细胞直径明显小于对数生长期的细胞(20, 19VS 36μm,P<0. 01)。血清饥饿的成纤维细胞,PCNA单抗染色阴性,证明离开了细胞周期,进入G0期,该期的细胞仍保持着活力。 |
| 关键词: 成纤维细胞,体外培养,血清饥饿,兔 |
| DOI： |
| 分类号:S814.6 |
| 基金项目:农业部生物技术重点项目(95牧-01-04-01) |
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| The impact of serum starvation on growth of rabbit fibroblasts |
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| Abstract: |
| After using DMEM including 0. 5% NCS for direct starvation, the
numbers of fetal and skin fibroblasts first increased slightly, then gradually decreased.
During the serum gradient starvation,with changing DMEM including 4%, 2% and 1%
NCS, the numbers of fetal and skin fibroblasts still increased a little; and after being
changed medium including 0. 5% NCS, the numbers gradually decreased.Diameter of the
fibroblast was obviously smaller than that of cellsduring log phase (20μm, 19μm VS 36
μm,P<0. 01) after serum starvation. Stained by antiproliferating cell nuclear antigen
(PCNA), the cells after serum starvation were negative. This confirmed the cells had
exited from cellcycle and been induced to G0 phase.The cells in G0 phase still maintained
viable. |
| Key words: fibroblast,culture in vitro,serum starvation,rabbit |
