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葡萄酒中白藜芦醇的HPLC测定
王 华1, 尉亚辉2, 王庆俐3
1.西北农业大学 葡萄酒学院;2.西北大学 生物技术重点实验室;3.西北农业大学 实验中心色谱组
摘要:
采用高效液相色谱(HPLC)法测定葡萄酒中白藜芦醇(T-RESVERATROL)的含量。葡萄酒样品经0. 45μm超滤后可直接进样测定;采用乙晴-水流动相进行1∶9~9∶1梯度洗脱,在波长306 nm下检测。试验结果表明,供试酒样中干红葡萄酒中白藜芦醇含量为0. 631 9~1. 881 8 mg/L,高于干白葡萄酒中白藜芦醇的含量(0. 127 1~0. 255 9mg/L)。
关键词:  干白葡萄酒  干红葡萄酒  白藜芦醇  HPLC
DOI:
分类号:TS262.6
基金项目:
Aualysis of Trans-Resveratrol in Wines by Direct Injection of HPLC
Abstract:
The content of resveratrol in wines was dedermined by HPLC.After being ultrafiltrated (0. 45μm), the wine samples were gradiently eluted by acetonitrile-water from 1∶9-9∶1.Then the content of resveratrol was detected under 306 nm.The results showed that among the wine samples, the resveratrol content in red wine was 0. 631 9~1. 881 8mg·L-1, higher than that of white wine (0. 127 1~0. 255 9 mg·L-1).
Key words:  dry white wine,dry red wine,resveratrol,HPLC