| 摘要: |
| 用限制性内切酶EcoR Ⅰ和Hind Ⅲ,从克隆载体pUTS Ⅱ中切出一个0. 988 kbTSPG Ⅱ基因,经EcoR I和BstX I酶切鉴定,同时表达载体也用相同的酶进行酶切,经琼脂糖凝胶电泳,分别回收TSPG Ⅱ基因和pSV·SPORT Ⅰ表达载体,用T4DNA连接酶定向连接,转化大肠杆菌感受态细胞,挑取重组子,经EcoR Ⅰ,BstX Ⅰ和Hind Ⅲ酶切鉴定,构建了重组表达质粒pSV·TS Ⅱ. |
| 关键词: 旋毛虫 ES抗原 结构基因 重组表达质粒 |
| DOI： |
| 分类号:S852.7 |
| 基金项目:农业部“八五”生物技术项目(85-04-03) |
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| Construction of Recombinant Expression Plasmids ofStructuralGene TSPGⅡ Encoding ES Antigenfrom Trichinella Spiralis |
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| Abstract: |
| Cloning vector pUTSⅡ was digested by Cloning vector pUTSⅡwas digested by EcoRⅠand HindⅢ and
eletrophoresed in agarose gel. The 0. 988 kb TSPGⅡ gene fragementwas recovered by
three methods. TSPGⅡ gene was inserted into vector pSV·SPORTⅠ which was
predigested by EcoRⅠand HindⅢ, and ligased to the downstream of SV40 promoter.
Thus recombinant expression plamids pSV·TSⅡ was constructed by T4DNA ligase.
Then this plasmids were transformed into E.coliDH5α and identified by EcoRⅠ,BstXⅠ
and HindⅢ.The recombinant expression plasmids of pSV·TSⅡ gene were constructed. |
| Key words: Trichinella spiralis,ES antigen,structural gene,recombinant expression plasmids |
