| 摘要: |
| 首次在国内建立了布尔山羊的细管精液冷冻程序。试验证明,选用30 g /L的葡萄糖、13 g/L的柠檬酸钠和1 g/L的乙二胺四乙酸钠(EDTA)作基础稀释液,乙二醇作保护剂,精液冻前处理后装入0. 5mL细管,在4℃下平衡1. 5 h,再以11～30℃/min的速率降温至-80℃以下(3～5 min)投入液氮,可获得良好的冷冻效果。用乙二醇作保护剂的冷冻精液,解冻后在12～15℃的常温下放置24 h,其活力仍可满足输精的要求。 |
| 关键词: 布尔山羊，细管，精液冷冻 |
| DOI： |
| 分类号:S827.03 |
| 基金项目:陕西省科委“九五”攻关项目(97K06-G2) |
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| A Study of Straw Frozen Sperm in Boer Goats |
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| Abstract: |
| The freezing of Boer Goat semen in straw was studied and the first freezing procedure in China established.The basic solution contained 30 g/L glucose, 13 g/L sodium citrate and 1 g/L ethylenediaminetetraacetic acid (EDTA). Sperm was treated
with a basic solution containing 100 mL/L egg yolk and centrifuged for removing sperm
serum before freezing, and was loaded into 0. 5mL straw placed in a 4℃ condition for
1. 5 h|.The cooling rate was about 11-30℃/min from 4℃ to -80℃, and then liquid
nitrogen was plunged in. The post-thaw sperm viability was 0. 3 to 0. 5 in 12 to 15℃
condition for 12 to 24 h and its viability could stillbe used in insemination. |
| Key words: Boer Goat,straw,sperm freezing |
