| 摘要: |
| 传染性法氏囊病Vero细胞灭活疫苗的免疫试验表明,用0. 5和0. 8 mL/只剂量分别肌肉接种。10～14日龄首免, 8 d后可检出病毒特异性抗体, 14 d抗体P/N值为3. 8～5. 6.病毒中和抗体滴度为1∶32～1∶64; 21 dP/N值为6. 3～6. 8,中和抗体滴度平均为1∶1 024～1∶2 048,此时血清抗体对鸡的保护率为85. 7%. 30～35日龄二免, 14 d后P/N值5. 0～5. 2,中和抗体滴度高达1∶2 048～1∶8 192,对鸡的保护率为100%.二免后112 d(147日龄)抗体仍保持较高水平,P/N值为3. 2～3. 4.野外试验表明,这种疫苗安全、有效。在3 000只蛋鸡群平行试验,灭活苗比活苗抗体水平高出0. 2个光密度值,抗体持续期长。接种灭活苗的鸡群5个月内未发生此病。 |
| 关键词: 传染性法氏囊病，细胞灭活苗，免疫试验，野外试验 |
| DOI： |
| 分类号:S855.3 |
| 基金项目:陕西省科委资助项目(95K02-G1-01) |
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| Vero Cell Culture Inactivated Virus Vaccine of IBD |
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| Abstract: |
| The research results of immunovaccination and field tests of IBD Vero
cell culture inactivated virus vaccine has been reported.The results showed that the vaccine had a quality immunogenicity when chicken was given 0. 5mL or 0. 8mL dosage by
subcutanous or muscular route. The date of vaccination first was conducted at 10-14
days of age. 8 d later, the chickens could be detected specific antibodies against IBDV and
P/N value detected by ELISA was 3. 8-5. 6 at 14 d, and titers of virus neutnaligation
(VN) antibody were 1∶32-1∶64.After 21 d,P/N value was 6. 3-6. 8, and titer of
VN antibody was 1∶1 024-1∶2 048.At this time, protective rate of sera for chicken against virulent IBD virus challenge was 85. 7 percent.Second vaccination was conducted
at 30-35 days of age.After 14 d of immunigation, the P/N value of of antibodies in sera
was 5. 0-5. 2, and titer of VN antibody was up to 1∶2 048-1∶8 192, and protective
rate of sera for chickens against virulent IBDV challenge was 100 percent.After 112 d of
second vaccination, the chicken had already been 147 days of age and still maintained
high level of antibodies and the P/N value was 3. 2-3. 4. Field tests showed that the
IBD Vero cell inactivated vaccine was salty and efficient. |
| Key words: IBD Vero, cell culture inactivated vaccine,immunovaccination,field tests |
