| 摘要: |
| 采用体外培养山羊睾丸间质细胞的方法,应用IGF-Ⅰ和hCG进行不同处理,利用放射免疫分析法(RIA)测定睾酮含量。结果表明:①30～40日龄山羊睾丸间质细胞适宜作为体外培养的材料;②山羊睾丸间质细胞的敏感性随hCG的重复刺激而下降;③IGF-Ⅰ能够促进睾酮的分泌(P<0.05～P<0.01);④IGF-Ⅰ和hCG对睾酮的分泌具有协同促进作用(P<0.05～P<0.01)。 |
| 关键词: 山羊，睾丸间质细胞，睾酮，IGF-Ⅰ，hCG |
| DOI： |
| 分类号:S814.1,S827 |
| 基金项目:国家自然科学基金资助项目 |
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| Effects of IGF-Ⅰand hCG on Testosterone Secretion in Cultured Goat Leydig Cells |
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| Abstract: |
| With the method of in vitro goat leydig cell culture and different treatments of IGF-Ⅰand hCG, the contents of testosterone have been determined by RIA.
The results showed that: (1) Leydig cells from 30～40-day-old goats are appropriate
materials for in vitro study. (2) The goat Leydig cell was sensitive to hCG stimulation
on testosterone production, but the sensitivity declined with the repeated hCG treatments. (3) IGF-Ⅰenhanced both basal and hCG induced testosterone secretion (P<0.05～P<0.01). (4) hCG and IGF-Ⅰhad accelerative function to testosterone secretion
(P<0.05～P<0,01). |
| Key words: goat, leydig cell, testosterone, IGF-I, hCG |
