摘要: |
对T型和V型小麦细胞质雄性不育系mtDNA的RAPD扩增产物分析结果表明,这两种不育系和其保持系间mtDNA的RAPD扩增产物存在着显著差异,而两种保持系间的扩增产物则无明显不同,说明小麦细胞质雄性不育可能与mtDNA的变异有关;同时,在两种不育体系的RAPD扩增产物之间,既有共性,又有差异,暗示这两种不育体系产生不育的机理可能不尽完全相同,而各具其特异性。 |
关键词: 小麦,细胞质雄性不育,mtDNA,RAPD |
DOI: |
分类号:S512.103.51 |
基金项目:国家攀登计划项目 |
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RAPD Analysis of mtDNA from Two Types of Cytoplasmic Male Sterility in Wheat |
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Abstract: |
The differences of mtDNAs between V-type and T-type CMS systems in
wheat were studied with RAPD technique.The results indicated that there were significant differences between the two male sterile lines and their maintainers in amplified
DNA fragments of mtDNA, and there were no differences between the two maintainers
in those of mtDNAs. It can be concluded that the cytoplasmic male sterility might have
something to do with variation of mtDNA. In addition, both similarities as well as differences were expressed on the polymorphism products between the two CMS systems. It
implied that the mechanisms of the CMS in wheat might be incompletely identical.The
results confirmed the conclusion from the RFLP analysis before. |
Key words: wheat (Triticum destivum), cytoplasmic male sterility(CMS),mitochondrial,DNA (mtDNA) |