| 摘要: |
| 本研究旨在证实牛体外培养的雄性胚胎生长发育是否快于雌性胚胎。 胚胎的性别鉴定采用多聚酶链式反应 (PCR)技术。 在体外受精的第 5天 ,胚胎根据发育期划分为三个组: 16细胞期 ,多于 16细胞期和桑椹期。 在 3个组中 ,雄性胚胎所占百分率分别是 32% ,53%和 63%。 在体外受精的第 7天 ,胚胎根据发育期划分为 4个组: 桑椹期、早期囊胚期、囊胚期和扩张囊胚期。 在性别鉴定后 , 4个组中雄性胚胎所占百分率分别是 28% , 38% , 60%和70%。 结果表明: 牛早期胚胎在体外培养条件下 ,雄性发育快于雌性。 |
| 关键词: 牛 ,胚胎 ,体外发育 ,雄性 ,雌性 |
| DOI： |
| 分类号: |
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| Sex Ratio of Different Developmental Stages in Vitro Produced Bovine Embryos |
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Li Qingwang 1 Liboriussen T 2
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| Abstract: |
| The objective of this study was to determine whether the bovine male embryos develop faster in vitro than female embryos. The sex of embryos were determined with PCR(Polymerase chain reaction) technique. On day 5(in vitro insemination= day 0), the
embryos were divided by developmental stages into 3 groups: 16 cells, more than 16 cells
and morula. In 3 groups, the percentages of males were respectively 32% , 53% and 63% .
On day 7(in vitro insemination= day 0) , the embryos were classified by developmental stages
into 4groups:morulae, early blastocysts, blastocysts and full-expanding blastocysts. After
sexing, the percentages of males were 28% , 38% , 60% and 70% , respectively. The study
demonstrated that IV F bovine male embryos develop faster in vitro than female embryos. |
| Key words: bovine, embryo, in vitro development, male, female |
