| 摘要: |
| 根据环核苷酸理化性质及乳的特点,采用酶法从乳汁中提取环核苷酸。研究表明,该方法条件温和、工艺合理、操作方便。经放射免疫测定提取浓缩液浓度为cAMP 20μmol·L-1, cGMP 0. 2μmol·L-1. |
| 关键词: 乳,环核苷酸,酶法 |
| DOI: |
| 分类号:Q524.03 |
| 基金项目:陕西省“八五”攻关资助项目及西北农业大学青年基金资助项目 |
|
| Extraction Method of Cyclic Nucleotieds of Milk |
|
Ma Zhike Zan Linsen Wang Qing Zhan Enpin Sun Chao,Ma Zhike Zan Linsen Wang Qing Zhan Enpin Sun Chao,Ma Zhike Zan Linsen Wang Qing Zhan Enpin Sun Chao
|
| Abstract: |
| Based on physical and chemical properties of cyclic nucleotydes and the
speciality of milk, cyclic nucleotides in milk were extracted with enzyme method. The
study showed that the method was moderate in condition, reasonable in technique and
easy in operation. The extracted fluid concentration of cAMP and cGMP were, by radioimmunoassay detction, 20μmol·L-1 and 0. 2μmol·L-1 respectively. |
| Key words: milk, cyclic nucleotides, extraction method |