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乳中环核苷酸提取方法研究
马志科1, 昝林森1, 王 倩1
西北农业大学 动物科学系
摘要:
根据环核苷酸理化性质及乳的特点,采用酶法从乳汁中提取环核苷酸。研究表明,该方法条件温和、工艺合理、操作方便。经放射免疫测定提取浓缩液浓度为cAMP 20μmol·L-1, cGMP 0. 2μmol·L-1.
关键词:  乳,环核苷酸,酶法
DOI:
分类号:Q524.03
基金项目:陕西省“八五”攻关资助项目及西北农业大学青年基金资助项目
Extraction Method of Cyclic Nucleotieds of Milk
Ma Zhike Zan Linsen Wang Qing Zhan Enpin Sun Chao,Ma Zhike Zan Linsen Wang Qing Zhan Enpin Sun Chao,Ma Zhike Zan Linsen Wang Qing Zhan Enpin Sun Chao
Abstract:
Based on physical and chemical properties of cyclic nucleotydes and the speciality of milk, cyclic nucleotides in milk were extracted with enzyme method. The study showed that the method was moderate in condition, reasonable in technique and easy in operation. The extracted fluid concentration of cAMP and cGMP were, by radioimmunoassay detction, 20μmol·L-1 and 0. 2μmol·L-1 respectively.
Key words:  milk, cyclic nucleotides, extraction method