| 摘要: |
| 应用萘酯坚固蓝反应显色法对DNA的酯酶催化活性进行了研究。结果表明,辣椒DNA、鲱鱼精DNA具有分解萘酯的活性;反应混合物中鲱鱼精DNA浓度为0. 0034mg /mL时,DNA仍有分解萘酯的活性;随着DNA浓度增加,DNA的催化活性增强。6种寡聚核苷酸均具有催化萘酯水解的能力,但活性强弱不同; 4种dNTP均不能催化萘酯水解。双链DNA、单链DNA的显色反应完全来自DNA的催化萘酯水解反应。 |
| 关键词: DNA，乙酸－α－萘酯，催化活性 |
| DOI： |
| 分类号:S641.303.2 |
| 基金项目:热带作物生物技术国家重点开放实验室课题资助项目 |
|
| Studies on the Esterase Catalytic Activity of DNA Molecules |
|
Wang Deyuan 1 Han Xinmin 2 Liu Zhixin 3 Peng Shiqing 3 Wang Ming 2 Zheng Xueqin 3
|
| Abstract: |
| The esterase catalytic activity of DNA molecules was studied using the
coloring-reaction of α-naphthyl acetate and fast blue RR.The results indicated that DNA
extracted from hot pepper,Capsicum annuum L., and DNA from herring sperm had the
activity of decomposing naphthyl acetate.When the concentration of herring sperm DNA
was 0. 0034mg /mL, DNA still possessed the catalytic activity.The esterase activity of
DNA increased along with the concentration of DNA. 6 kinds of oligonucleotide also
showed catalytic activity to hydrolyz enaphthyl acetate, but differed in the intensity of
activity. 4 kinds of dNTP could not catalyze the hydrolysis of naphthyl acetate at all.
The coloring-reaction of dsDNA and ssDNA in the reaction mixtures resulted wholely
from the hydrolysis, catalyzed by DNA, of naphthyl acetate. |
| Key words: DNA,α-naphthyl acetate, catalytic activity |
