| 摘要: |
| 通过对CaMV CABB-BJI和Bari-1株系基因Ⅵ的克隆和转化,在根癌农杆菌菌种GV3101的介导下,利用真空渗入法获得了转化CABB-BJI和Bari-1基因Ⅵ的拟南芥转基因植株。分子检测表明,基因Ⅵ已整合进拟南芥基因组并有不同程度的表达。在转基因植株中,出现了类似病毒感染症状、多生长点及花粉生活力较低等个体的变异株。而多数转基因植株(占转基因植株70. 4% )为无症状的正常植株。对转化CaMV Bari-1株系基因Ⅵ的9个无症状转基因株系接种CaMV CABB-BJI病毒抗性表明, 9个转基因株系表现出了不同的抗性。 |
| 关键词: CaMV基因Ⅵ，遗传转化，转基因植株表型，遗传工程的交叉保护，拟南芥 |
| DOI： |
| 分类号:Q785 |
| 基金项目: |
|
| The Genetic Transformation and Genetically Engineered Cross Protection of CaMV Gene Ⅵ in Arabidopsis Thaliana |
|
Gong Zhenhui 1 Milner J J 2 He Yuke 3 Chen Qilin 1 Xue Wanxin 1
|
| Abstract: |
| Agrobacterium tumefaciens strain GV3101 mediated transgenic plants
containing CABB-BJI gene Ⅵ and Bari-1 gene Ⅵ were obtained in Arabidopsis thaliana
by using the method of vaccum infiltration through the cloning and transformation of
gene Ⅵ of cauliflower mosaic virus (CaMV) strains CABB-BJI and Bari-1.The molecular assay showed that the gene Ⅵ was integrated into plant genomes of Arabidopsis thaliana and had expressions of various degrees.The mutants of different types, including virus symptom-like phenotype,multiple growing points and lower viability of pollen,
were found among the transgenic plants.Most of the transgenic plants (70. 4 percent),
however,were symptomless. The strain CABB-BJI of CaMV artificially inoculated nine
lines of symptomless transgenosis from the transformed gene Ⅵ of CaMV strain Bari-1.
The results of the resistance indicated that the nine lines had various resistance reactions. |
| Key words: geneⅥ of CaMV, genetic transformation, genotype of transgenic plants, genetically engineered cross-protection,Arabidopsis thaliana |
