| 摘要: |
| 用随机扩增多态型DNA(RAPD)技术,在幼苗期鉴定了圆叶葡萄和真葡萄亚属杂交的杂种。结果表明,以两个杂交组合后代及其亲本DNA作模板,用随机引物UBC-250,UBC-266和UBC-272可以分别扩增出1400, 1440, 350和500 bp的多态性片段,作为来自无核亲本的分子标记。 |
| 关键词: RAPD，圆叶葡萄，葡萄杂种，分子标记 |
| DOI： |
| 分类号:S663.103.2 |
| 基金项目:USDA资助项目的部分内容 |
|
| Identification of Hybrids Derived from Vitis rotundifolia×Vitis vinifera by RAPD Analysis |
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Wang Yuejin 1 Lamikanra O. 2 Schell L. 2 Lu Jiang 2
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| Abstract: |
| The identification of hybrids derived from Vitis rotundifolia×Vitis vinifera cultivar was carried out using RAPD techniques during seedling stage. The results showed the DNA polymorphisms of 1400, 1440, 350 and 500 bp were amplified by
random primers of UBC-250, UBC-266, and UBC-272 and targeted to the molecular
markers from seedless parents when the hybrids DNA were used as the templates from
two cross combinations. Therefore, the hybrids, of both true and false would be determined by these molecular genetic markers before fruit production. |
| Key words: RAPD,Vitis rotundifolia,Vitis hybrid,molecular genetic marker |
