| 摘要: |
| 通过水平薄层变性聚丙烯酰胺凝胶等电聚焦(IEF)、十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)和双向电泳(IEF×SDS-PAGE)研究表明,干旱引起小麦幼芽蛋白质组分及含量变化,主要在分子量为20～48 kD、等电点(pI)为5. 34～5. 65,并且干旱诱导小麦幼芽产生41. 5 kD蛋白亚基, pI为5. 65;分子量为36. 2(pI 5. 35)和15. 8 kD(pI 5. 70)的两个蛋白亚基含量明显增加。 |
| 关键词: 小麦幼芽，干旱，蛋白质组分，等电聚焦，双向凝胶电泳 |
| DOI： |
| 分类号:Q945.17 |
| 基金项目:农业部“八五”重点研究课题 |
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| Effect of Osmotic Stress on Isoelectric Point andSubunit Composition of Proteins in Shoots of Wheat |
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| Abstract: |
| Studies of proteins in shoots of wheat with isoelectric focusing, sodium
dodecylsufate-polyacrylamide gel electrophoresis ( SDS-PAGE ) and two-dimensional
electrophoresis ( IEF×SDS-PAGE )show that changes in protein profiles and level of
protein occurmainly with the molecular weight of 20～48 kD, pI values of 5. 34～5. 65
and osmotic stress, and the predominant protein ( subunit ) with apparent MW of 41. 5
kD can be induced also. While, the level of two proteins [36. 2 kD (pI5. 35)、15. 8
kD ( pI 5. 70)] increased greatly. |
| Key words: shoot of wheat, osmotic stress, protein composition, isoelectric focusing, two-dimensional electrophoresis |
