| 摘要: |
| 不同品种大蒜,蒜瓣分化新梢的途径和能力差异较大。山东苍山大蒜蒜瓣经35℃贮藏5个月,直接分化新梢的效果较好;初次培养第28 d切割分化的叶片(蒜瓣)分化新梢的数量比其他时间切割均多,细胞分裂素选用0.1 mg/L ZT并配合0.005 mg/L的NAA加入MS基本培养基时,单瓣蒜繁殖系数最高。培养基中加入青霉素不仅可以减少污染,而且能促进新梢的分化。 |
| 关键词: 大蒜，离体繁殖，玉米素，贮藏温度 |
| DOI： |
| 分类号:S633.403.3 |
| 基金项目:国家自然科学基金资助项目 |
|
| A Study on the Effecting Factors of Garlic Propagation in Vitro |
|
GuoyiYangZhuying
|
| Abstract: |
| Cloves of different garlic cultivars have different capacities to produce
shoots in vitro by different ways.Cangshan garlic cloves can produce high quality shoots
directly after stored at 35℃ for five months before being cultured in vitro.Cutting leaves
when the cloves were cultured for 28 days,the explants could produce more shoots than
that at other times.The propagation index per clove was the highest when cloves of
Cangshan garlic were cultured on MS medium supplemented with 0.1 mg/L ZT and 0.
005 mg/L NAA.With penicillin sodium added into the medium,contamination was
suppressive and cloves were promoted to differentiate new shoots. |
| Key words: garlic,propagation in vitro,storage temperature,ZT |
