| 摘要: |
| 从低温(—20℃)贮藏的玉米花粉中分离出的生活精细胞,其产量随贮藏时间的延长而降低。但是,从-20℃贮藏24 h以内的花粉,仍可分离到相当数量的生活精细胞。以新鲜花粉作对照,从贮藏8 h的花粉分离生活精细胞,相对产量可达64%. |
| 关键词: 纯系玉米，花粉，低温贮藏，生活精细胞 |
| DOI： |
| 分类号:Q94-33 |
| 基金项目:国家自然科学基金资助项目 |
|
| The Isolation of Viable Sperm Cells from Cryopreserved Pollen of Zea mays L. |
|
Xu Hengping Cao T. H.
|
| Abstract: |
| Although the yield of viable sperm cell of Zea mays L.decreases with the
cryopreserving time of pollen at -20℃,certain amount of the viable sperm cells could
still be obtained from the frozen pollen within 24 hours.The relative sperm yield from
the pollen stored at -20℃ for 8 hours could reach about 64%,compared with that from
fresh pollen. |
| Key words: Zea mays L.,inbred line,pollen,cryopreservation,sperm cells |
