| 摘要: |
| 采集繁殖和非繁殖季节山羊卵巢，回收大腔（3-6mm）和小腔（<2mm）卵泡卵母细胞，进行体外成熟和受精，结果如下：①繁殖季节的大腔和小腔卵泡卵母细胞体外成熟率（68.4%．，27.5%）均显著（P<0.05）高于非繁殖季节（52.5%，20.6%）；②1和1.5周岁龄羊与成年羊相比，前两者的小腔卵泡卵母细胞采集数显著（P<0.05）多于后者（25.6,22.5：15.1），但卵母细胞体外成熟率无显著差异（P<0.05）；③随卵母细胞直径增加，其体外成熟率呈显著上升趋势；④与加新生犊牛血清和生殖激素在颗粒细胞悬浮液中培养相比，加发情山羊血清和激素在颗粒细胞单层上培养可显著（P<0.05）提高小腔卵泡卵母细胞体外成熟率（20.8%,34.5%）；⑤将体外成熟的小腔卵泡卵母细胞与体外获能精子进行受精，其受精率为35.7%（51/143）。 |
| 关键词: 小腔卵泡，卵母细胞，体外成熟，体外受精，山羊 |
| DOI： |
| 分类号:S827 |
| 基金项目:国家自然科学基金资助项目和农业部“八五”攻关项目 |
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| Maturation and Fertilization in Vitro of Goat Oocytes Derived from Small Antral Follicles |
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| Abstract: |
| Goat ovaries were collected at breeding and nonbreeding seasons.The
oocytes derived from large (LAF,3一6mm) and small antral follicles(SAF,<2mm)
were recovered and used for maturation and fertilization in vitro.The results are as follows;① in vitro maturation rate of LAF and SAF oocytes is significantly higher at
breeding seasons (68.4%,27.5%) than at nonbreeding seasons (52.5%,20.6%);②
the numbers of SAF oocytes colleeted from the ovaries of goats at l (25.6)
and 1.5 (22.5) years old is more than that from adult goat ovaries (15.1),but there is
no significant difference in oocyte maturation rates among three groups;③in vitro maturation rates of oocytes increases significantaly with their diameter increase;④more high
maturation rates of SAF oocytes is obtained when culture in medium with GS,FSH,LH
and 17β一E2 on granulosa cell monolayer (34.5%) in comparison with culture in granulosa cell solotion with NCS,FSH,LH and 17β一E2 (20.8%);⑤SAF oocytes maturated
were fertilization in vitro with capacited sperm and in vitro fertilization rate is 35.7%
(51/143). |
| Key words: small antral follicle,oocyte,in vitro maturation,in vitro fertilization,goat |
