| 摘要: |
| 利用生物统计方法对用于低硫苷油菜育种后代材料筛选、定量的的氯化钯—分光光度法操作的可靠性及适用条件进行了分析，结果表明：①采用１次重复测定可对硫苷含量差值为０．０４８ｍｇ／ｇ的种子样品作出正确的分辨，用于育种后代材料筛选；②采用２次重复测定的平均值可对油菜—类种子的硫苷含量给出较准确的估计。 |
| 关键词: 油菜，硫苷分析，氯化钯—分光光度法 |
| DOI： |
| 分类号:S565.401 |
| 基金项目:陕西省“八五”攻关项目 |
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| Determination of Glucosinolate Content in Rapeseed with Palladium Chloride-Spectrometry Method |
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| Abstract: |
| The reliability of palladium chloride-spectrometry method and its application condition in material screening with low glucosinolate in rapeseed breeding havebeen discussed with statistical method in this paper.The results show that:1.determination with one repeatation can produce a correct differentiation for total glucosinolatecontent of Brassicas oil seeds with different values of 0.048 mg/g for the materialscreening in early generation;2.average value determined with two repeatations can result in an accurate estimate for Brassicas oil seed. |
| Key words: repeseed,glucosinolate analysis,palladitim chloride spectrometry |
