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油菜、荞麦根分泌物中糖及有机酸组分的研究
王俊儒1, 安保珠1, 尉庆丰2
1.西北农业大学 基础科学系;2.西北农业大学 农化系
摘要:
利用连续流动收集系统收集了油菜、荞麦根分泌物,对介质pH变化、分泌物中碳水化合物组成及有机酸进行了测定。结果表明,荞麦、油菜生长介质中pH值变化的差异反映了它们泌质子能力的差异。在油菜分泌物中检测出高水平的鼠李糖,在荞麦中则是阿拉伯糖含量最高。草酸、琥珀酸是两种作物根分泌物的主要有机酸。苹果酸、柠檬酸在油菜根分泌物中没有检测出。
关键词:  根分泌物,pH,有机酸,碳水化合物,油菜,荞麦,HPLC
DOI:
分类号:S311
基金项目:国家自然科学基金资助项目
Determination of Carbohydrates and Organic Acids in the Root Exudates of Rape and Buckwheat
Abstract:
The pH value changes in the rooting medium,composition of carbohydrates,and organic acids of the rape and buckwheat root exudates,got with continuous flowing collecting system,were determined.The results show that the different changes of pH values reflect the different H+-excetion ability in the two crops.High level of Rhamnose was found out from the root exudates of rape while high level of Arabinose from buckwheat.Oxalic and Succinic acids were the main organic acids in the root exudates from both crops.No malic and citric acids were found in rape cxudates.
Key words:  root exudate, pH value,organic acids,carbohydrates,rape,buckwheat,HPLC