| 摘要: |
| 试验在Ｗｈｉｔｅ培养基上获得了最好的结果，其次是Ｈｅｌｌｅｒ和Ｎｉｔｓｃｈ培养基，在ＭＳ培养基上杂种子房发育最差；添加改良Ｗｈｉｔｅ培养基附加维生素可明显促进子房的发育和杂种种子的形成，增加生物素可进一步促进Ｗｈｉｔｅ培养基维生素作用的发挥，但肌醇和泛酸则会抑制ｗｈｉｔｅ维生素的作用；杂种子房发育的培养基蔗糖浓度范围为２５～５０ｇ／Ｌ，以５０～７５ｇ／Ｌ为宜；白菜花器提取液或水解酪蛋白均对子房发育有促进作用，但并非必要条件。 |
| 关键词: 白菜，白芥，远缘杂交，离体子房培养 |
| DOI： |
| 分类号:S634.035 |
| 基金项目:国家自然科学基金资助项目 |
|
| Effect of Culture Medium on the Development of Excised Hybrid Ovaries between Brassica campestris and Sinapis alba |
|
Gong Zhenhui He Yuke Wang Ming Wang Xiaofeng
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| Abstract: |
| Three different results of the excised hybrid ovaries from the best to the
worst were obtained on the White’s mineral,the Heller’s mineral and the Nitsch’s mineral as well as the Murashige and Skoog’s mineral,respectively.Modified White’s vitamins added on the tested culture media could accelerate the development of the excised
ovaries and the hybrid seeds distinctively.The modified White’s vitamins plus biotin
could further accelerate the function of the modified White’s vitamins.However,the
modified White’s vitamins plus myo-inositol or pantothenate could lower the function of
the modified white’s vitamins on the development of the excised ovaries.Sucrose concentration extent was 25～150 g/L for the growth and development of the excised ovaries.The effect of the extraction solution from Chinese cabbage flower organ(500 ml/L)or casein hydrolysate(300 mg/L)in the medium on the development of the excised
ovaries was favourable,but not indispensable in culture in vitro of the excised ovaries. |
| Key words: Chinese cabbage,white mustard,distant hybridization,excised ovary culture |
