| 摘要: |
| 利用聚丙烯酰胺凝胶电泳（ＰＡＧＥ）方法和区带活力扫描技术，分析了２１１头黑白花奶牛乳中ＬＤＨ的多态性及其活力与某些产奶性能的关系。结果表明，乳中ＬＤＨ同工酶谱带与其他动物相似，并以Ｂ型酶占优势。ＬＤＨ1酶带的活力最高，同３０５ｄ产奶量呈极显著相关关系（ｒ＝０．８９０３）；ＬＤＨ5的活力与乳脂率和３０５ｄ产奶量存在显著的正相关（ｒ＝０．７８３５）及负相关（ｒ＝－０．７０３２）．方差分析发现，ＬＤＨ型和胎次对３０５ｄ产奶量和乳脂率有显著或极显著的影响作用，ＡＡ型的最高，各型产奶量大小依次为：ＡＡ＞ＡＤ＞ＡＢ＞ＡＣ＞ＡＥ。 |
| 关键词: LDH同工酶，牛乳，产奶性能 |
| DOI： |
| 分类号:S823.91 |
| 基金项目:高等学校博士点基金资助项目 |
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| Study on the Characters of LDH Isoenzymes in Cow's Milk |
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Luo Jun Qiu Huai Wang Shuanghe
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| Abstract: |
| The relationships between milk producing traits and characteristics of LDH isoenzymes,including its polymorphisms and relative activity in cow's milk, were analysed by using a combination of PAGE and spectrophotometric scanning.The result showed that the spectrum of the LDH isoenzymes in cow milk demonstrated a similar pattern with tissues of other animals and B-type had an advantage in contents over Atype. The elative activity of LDH1,the highest among all bands of the LDH enzymes,had a significant association with 305 d milk yield , while the activity of LDH5 showed positive and negative correlations with fat percentage and 305 d milk yield, respectively.The LDH types and parities exhibited a marked effect on the 305 d milk yield and fat percentage by ar analysis of variance. |
| Key words: LDH isoenzymes ,cow's milk,milk producing traits |
