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烟草花叶病毒诱发抗性的研究
黄丽丽1, 魏宁生1
西北农业大学 植保系
摘要:
用烟草花叶病毒(TMV)接种过敏性寄主枯斑三生烟或心叶烟的半叶或下部两叶,可诱导出未接种的另半叶或上部叶对TMV再次感染的抗性,诱发接种后7d用TMV进行攻击接种,所产生的枯斑直径明显小于对照。诱发抗性最早在诱发接种后3~4d被检测到,第7 d左右达到高峰,温度对诱发抗性的产生有很大影响。诱发接种叶上部的未接种叶因叶位不同,其抗性强弱也不同。经TMV诱发后的心叶烟可表现出对TMV和CMV两者的抗性,在攻击接种CMV后,潜育期比对照延长1~2d.
关键词:  烟草花叶病毒,心叶烟,枯斑三生烟,诱发抗性
DOI:
分类号:S435.72
基金项目:国家自然科学基金资助项目
Systemic Induced Resistance of Tobacco Mosaic Virus
Huang Lili Wei Ningsheng
Abstract:
Inoculation of half-leaves of lower 2 leaves of hypersensitive tobacco Nicotiana tobacum var. Samsun NN orNicotiana glutinosa,with tobacco mosaic virus (TMV)induce a high level of resistance to TMV in the opposite half-leaves or upper leaves.Challenge inoculation with TMV of the resistant half-leaves of upper leaves 7 days after the first inoculation resulted in limited lesion formation,the lesions were constantly smaller than those on the control plants.Temperature has a great effect upon the induced resistances.The strong and weak resistance of the uninoculated leaves in the upper of the induced inoculated leaves is different beeause of different leaf positions. The leaves ofN. glutinosa with TMV induced resistance were resistant not only to TMV but also to cucumber mosaic virus(CMV).The latent period of CMV in resistant leaves was l一2 days longer than that on the control plants.
Key words:  TMV,Nicotiana glutinosa,Nicotiana tobacum var.Samsun NN,induced resistance