| 摘要: |
| 将0.5％纤维素酶Rs+0.1％果胶酶Y-23酶溶液振荡处理3h,可得到最高的细胞分裂率,但原生质体分离数量较低。0.5％纤维素酶R-10+0.05％离析酶R-10+0.05％果胶酶Y-23酶溶液,25℃下处理14h,原生质体分离数量及细胞分裂率均较高。1/2MS培养基添加NAA3mg/L+BA0.5mg/L+2,4-D 0.1mg/L的液体培养基有利于细胞团形成及愈伤组织生长。本试验获得了完整的再生植株,芽分化率达20％左右。 |
| 关键词: 甘蓝,子叶,原生质体培养,液体培养基,植物激素/植株再生 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| The Culture of Cotyledon Protoplasts and Regeneration of Plantlets of Brassica oleracea |
|
Sun Zhenjiu
|
| Abstract: |
| Through the treatment of application of 0.5% cellulase Rs+0.1%
Pectolyase Y一23 by shaking for 3h,the highest cell division effieiency was obtained,
but the number of protoplast isolation was relatively low.Also,through the
treatments using enzy meinixture of 0.5% cellulase R-10+0.05% macerozyme
R-10+0.005% pectolyase Y-23 incubated at 25℃ for 14h,more protoplasts were
isolated and a high efficiency of cell division was gained.The liquid medium of l/2
MS containing 3mg/L NAA,0.5mg/L BA and 0.1mg/L 2,4-D was favourable
to the formation of cellular aggreate and the callus growth.Complete plantlets
regenerated were obtained,and the bud differentiation rate can reach 20% or so in
this experiment. |
| Key words: Brassica oleracea,Cotyledons,Protoplast culture,liquid media,phytohormones / plantlet regeneration |
