| 摘要: |
| 用荧光染料DAPI研究结果表明,小麦条锈菌夏孢子染核的最适浓度为5μg/mL,染色时间为3min;夏孢子芽管染核的适宜浓度为3μg/mL,染色时间为2～3min。本研究还发现DAPI对分属于5个亚门中的30种植物病原真菌的孢子(孢子囊)、芽管和菌丝同样具有较好的染核效果。 |
| 关键词: 小麦,双孢锈属,夏孢子,细胞核,染色(生物学)/条锈菌,芽管,病原真菌,荧光染色 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目 |
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| The Technique of Flouresence Staining Nuclei of Uredospore and Germ Tube of Wheat Stripe Rust |
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Rang Zhensheng Li Zhenqi Shang Hongsheng Ma Qing Wang Huiwen,Rang Zhensheng Li Zhenqi Shang Hongsheng Ma Qing Wang Huiwen,Rang Zhensheng Li Zhenqi Shang Hongsheng Ma Qing Wang Huiwen
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| Abstract: |
| Research results indicated that optimal staining concentration for uredospore was 5 ug/mL, and staining time is 3 min. and that optimal staining concentration for germ tube was 3 ug / mL, and the staining time 2 to 3 min. Furthermore, it was found in the study that the nuclei in spores (sporangia), germ tube and mycelia of 30 species of pathogenic fungi from 5 subdivitions could be successfully stained with DAPI. |
| Key words: Triticum aestivum,Puccinia,urediniospore,cell nucleus,staining (biology)/Puccinia striiformis West,germ tube,pathogenic fung,flouresence staining |
