| 摘要: |
| 对影响小麦花粉植株诱导频率部分因素进行了研究,获得以下结果:(1)在现有的小麦花药愈伤组织诱导培养基中,W14和马铃薯-Ⅱ的效果最好;(2)低温预处理的效果因杂交组合的不同而表现不同;(3)短期高温预培养可明显提高愈伤组织的产量;(4)绿苗分化率与愈伤组织年龄关系密切,愈伤组织转移的最适年龄与愈伤组织诱导培养基的种类有关;(5)大量元素减半的MS培养基绿苗分化率显著高于190—Ⅱ培养基。 |
| 关键词: 小麦,花粉植株,愈伤组织,诱导频率 |
| DOI： |
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| Studies on Improvement of Induction Frequency of Pollen Plantlets in Wheat |
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Hu Ling Xu Weigang Zhao Xiangke
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| Abstract: |
| The paper deals with the improvement of induction frequency of pollen plantlets in wheat with experimental results obtained as follows：( 1 ) W14 and potato-Ⅱ were the best to induce callus among current wheat anther media. ( 2 ) The effect of lower temperature pretreatment varied significantly with different cross combinations. ( 3 ) The yield of callus increased apparently by higher temperature preculture for a short time. (4) The differentiation frequency of green plan was closely related to the age of callus and its optimum age was different when varied callu induction media were used. (5) MS medium decreased half in thelevel of major elements was better than 190-2 medium for green plant differentiation. |
| Key words: wheat, pollent plantlet, callu, induction frequency |
