| 摘要: |
| 本试验用凝脉电泳法分析了44份甜瓜(Cucumis melo L.)材料功能叶的过氧化物酶(POD)、细胞色素氧化酶(Cyt)、儿茶酚酶(Cat)及酯酶(Est)同工酶和10个材料感病前后POD及Cat同工酶的变化。结果表明除Est外,不同材料的其他三种氧化酶均存在着明显的同工酶差异,其中POD同工酶的差异最大,Cyt次之,Cat相对较小。所分析的同工酶系均不能将薄皮甜瓜和厚皮甜瓜截然分开,这两类甜瓜在同工酶水平上亲缘关系很近。甜瓜植株感染病毒后其功能叶POD和Cat同工酶分子数量和种类明显增加。 |
| 关键词: 甜瓜,种质,同工酶,凝胶电泳,病毒病 |
| DOI： |
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| 基金项目: |
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| Electrophoretic Analysis of Isozymes in Cucumis melo L. Germplasm |
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Zhang Xingping Wang Ming
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| Abstract: |
| 44 varieties of Cucumis melo L, were analyzed for peroxidase (POD) , cytochrome oxidase (Cyt) .catechol oxidase (Cat) and esterase (Est) isozyme phenotypes, and of 10 varieties were assayed for comparison of PQD and Cat phenotype difference between healthy plants and virus infected plants of their functional leaves by using polyacrylamide gel electrophoresis in the present study. Considerable variation was observed between varieties in three oxidases, the greatest diversity in POD and followed by Cyt and Cat, except the Est isozyme. However, two large horticultural groups of Cucumis melo L. oriental melon and cantaloupe, could not be distinguished by specific isozyme phenotypes or characteristic bands of the enzyme assayed. Oriental melon and cantaloupe have a close relation in the enzyme level. Increased bands and darker stained bands were found on the plants infected by virus both in POD and Cat systems. |
| Key words: Cucumis melo,Germplasm,Isozyme,Electrophoretic analysis,virus diseases |
