| 摘要: |
| 本试验发现了乳汁中干扰因素对17β—雌二醇放射免疫测定方法的影响规律。在标准曲线中设立一个乳汁干扰因素对照点(N1 管),计算时用 N1 管的cpm(每分钟计数)减去与标准曲线 Bi 管(i=0,1,……6)条件一致的N2 管的 cpm,其差数就是乳汁干扰因素所造成的 cpm。将 cpm 加入标准曲线的 Bi 管中,这时 Bi 管内的 cpm〔 (N1一N2)〕与乳汁样品管的 cpm 条件一致。用标准曲线 Bi 管校正后的 cpm 计算出的回归方程,所求出的乳汁17β一雌二醇含量很接近其真实含量。 |
| 关键词: 乳汁,17β—雌二醇,放射免疫测定方法 |
| DOI： |
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| IMPROVEMENT OF RADIOIMMUNOASSAY OF ESTERADIOL-17β IN MILK |
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Zhang Yanming Gong Guangzhi Wang Jianchen,Zhang Yanming Gong Guangzhi Wang Jianchen,Zhang Yanming Gong Guangzhi Wang Jianchen
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| Abstract: |
| The law of interference factors affecting radioimmunoassay of estradiol-17β in milk was found in this experiment.A controlled point (N1) for interference factors in milk was set up at the same standard curve.In calculating,difference between cpm in N1 tubes (counted by per minute) minus cpm in N2 tubes with the same conditions an Bi tubes (i=0,1······6) at the standard curve was the cpm caused by interference factors in milk When this cpm was added to Bi tubes of standard curve,the cpm in Bi tube [ +(N1-N2) ] was agreeable with the same conditions of the cpm in milk sample tubes.Thus,concentration of estradiol-17β in milk measured by radioimmunoassay was almost near to its true concentration. |
| Key words: milk,estradiol-17β,radiommunoassay |
