| 摘要: |
| 孕酮是与生殖生理密切相关的雌性激素。1973年,Heap 在英国首次建立了乳汁孕酮的放射免疫分析法;1982年,国内建立了这种测定法。为了提高测定效率,经多次实验对该法进行了改进研究,取得了满意的效果。改进包括三个方面。第一,用等量的明胶磷酸盐缓冲液代替加入标准曲线管中的标准奶;第二,将温育温度由原来的4℃改为37℃;第三,将温育时间由原来的8～16小时缩短为20分钟。这些改进不但使所建立的标准曲线更加理想,而且缩短了测样时间。用本法测定的秦川牛和摩拉水牛产后未乳的孕酮水平和范型与文献报道相近似。 |
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| Modification of Radioimmunoassay of Progesterone in Milk |
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| Abstract: |
| The modification of radioirmmunoassay of progesterone in milk was described in this paper.It is different from the previous method in those, (1) standardized milk for standard curve tubes was replaced by equivalent gelatine phosphate buffer, (2) the temperature for incubation was changed from 4℃ to 37℃,and (3) the time for incubation was shortened from 8-16 hours to 20 minutes.The method modificated not only had an ideal standard curve,but also shortened the time for measurement.Levels of progesterone in the last milk of Qinchuan cattle and Murrah buffalo were measured by two methods.The levels and patterns of progesterone are similar to those reported in the literatures. |
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