| 摘要: |
| 海登汉氏苏木精法也称铁矶苏木精法,是常用的真菌细胞核染色方法,R.Litt-le & J.G.Manners(1969)曾报导用此法可对小麦条锈菌夏孢子芽管核进行染色,但 R.B.Volin(1971);井金学、商洪生(1980);李振岐、E.L.Sharp 和 M.Rein-hold(1983)在对小麦条锈菌夏孢子芽管核的染色试验中均发现用此法染核难以掌握。为此,李振岐和 E.L.Sharp 等于1983年3—4月结合小麦条绣菌异核作用研究需要对海登汉氏苏木精染色方法进行了改进研究。在反复试验中发现, 改用稀释乳酚油做分色剂, 并将分色步骤移到用桔红G 染色之后, 可比较容易地对小麦条锈菌夏孢子芽管核进行染色。1983年5 月-1984年8月李振岐、陆和平、马青等又在此基础上进行了补充改进试验, 着重研究了染色时间及本方法的适应范围和与此法配套的相应的制片技术等。 |
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| Studies on Modification of Haidenhains Haematoxylin Method for Staining Germ Tube Nuclei of Stripe Rust of Wheat |
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| Abstract: |
| Haidenhains Haematoxylin is widely used for staining nuclei of fungi.In 1969 R. Little and J. G. Manners reported that they could successfully stain germ tube nuclei of stripe rust by this method.And yet,according to the experience of R. B. Volin (1971),Jing Jinxue ,Shang Hongsheng(1981),Li Zhenqi and E.L. Sharp(1983),it is difficult to use this method to stain germ tube nuclei of stripe rust.For this reason in Feb.-Mar. 1983,Li Zhenqi and E.L.Sharp began to modify this method and found that taking dilute(adding 1/4-1/3 glycerin)lactophenol instead of 4 percent of ferric alum as the differenciator could easily stain germ tube nuclei of stripe rust,and then from May 1983 to April 1984,Li Zhenqi,Lu Heping,Ma Qing et al.continued to modify this method, and found that using this modified Haidenhains Haematoxylin method could successfully stain uredospore nuclei,if the uredospores used are just,ripe,and also found that the nuclei of more than 20 species of pathogenic fungi belonging to 5 suborders could be successfully stained by this method.Thus,we think that the modified Haidenhains Haematoxylin method can be considered as a simple,useful,and widely adaptable method for staining nuclei of fungi. |
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